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Pure Appl. Chem., 2012, Vol. 84, No. 10, pp. 2027-2043

Published online 2012-05-22

Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo1, Koji Nakano1, Hizuru Nakajima2, Katsumi Uchiyama2, Akihide Hemmi3, Yoshikazu Yamasaki4, Shigeharu Morooka4, Ryoichi Ishimatsu1 and Toshihiko Imato1*

1 Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, 819-0395, Japan
2 Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, Tokyo, 192-0397, Japan
3 Mebius Advanced Technology Ltd., Tokyo, 167-0042, Japan
4 Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Fukuoka, 814-0180, Japan

Abstract: A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti-APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H2O2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC80.