Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Guo, Shuai; Nakano, Koji; Nakajima, Hizuru; Uchiyama, Katsumi; Hemmi, Akihide; Yamasaki, Yoshikazu; Morooka, Shigeharu; Ishimatsu, Ryoichi; Imato, Toshihiko

doi:10.1351/PAC-CON-11-10-10

Pure Appl. Chem., 2012, Vol. 84, No. 10, pp. 2027-2043

http://dx.doi.org/10.1351/PAC-CON-11-10-10

Published online 2012-05-22

Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo¹, Koji Nakano¹, Hizuru Nakajima², Katsumi Uchiyama², Akihide Hemmi³, Yoshikazu Yamasaki⁴, Shigeharu Morooka⁴, Ryoichi Ishimatsu¹ and Toshihiko Imato¹*

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, 819-0395, Japan
² Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, Tokyo, 192-0397, Japan
³ Mebius Advanced Technology Ltd., Tokyo, 167-0042, Japan
⁴ Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, Fukuoka, 814-0180, Japan

Abstract: A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti-APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H₂O₂ and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC₈₀.