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Pure Appl. Chem., 2013, Vol. 85, No. 9, pp. 1771-1787

Published online 2013-07-22

NMR studies on carbohydrate interactions with DC-SIGN towards a quantitative STD analysis

Cinzia Guzzi, Juan Carlos Muñoz-García, Pedro M. Enriquez-Navas, Javier Rojo, Jesús Angulo and Pedro M. Nieto*

Glycosystems Laboratory, Department of Bioorganic Chemistry, Instituto de Investigaciones Químicas (CSIC), Avda. Américo Vespucio 49, Sevilla 41092, Spain

Abstract: The recent introduction of saturation transfer difference (STD) NMR has increased the tools for the study of protein–carbohydrate complexes. This is useful when it is combined with transfer nuclear Overhauser enhancement spectroscopy (NOESY) measurement, or when it is interpreted using the expected calculated values of transference, yielding additional, very valuable information for the study of this type of complex. The objective of this work is to cover the advances of the STD technique as exemplified by the investigations of DC-SIGN (dendritic cell-specific ICAM-3 grabbing non-integrin) recognition by simple carbohydrates or mimics of them, based on structures containing a terminal mannose or fucose. We also will discuss the methods for quantification of the STD values based on the initial growing rates with the saturation time.