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Pure Appl. Chem., 2013, Vol. 85, No. 10, pp. 2005-2013

http://dx.doi.org/10.1351/PAC-REP-12-03-03

Published online 2013-09-30

ANALYTICAL CHEMISTRY DIVISION

ORGANIC AND BIOMOLECULAR CHEMISTRY DIVISION

PHYSICAL AND BIOPHYSICAL CHEMISTRY DIVISION

Determination of the photoluminescence quantum yield of dilute dye solutions (IUPAC Technical Report)

Ute Resch-Genger* and Knut Rurack

Federal Institute for Materials Research and Testing (BAM), Division I.5, Richard‑Willstätter-Str. 11, D-12489 Berlin, Germany

Abstract: Procedures for the determination of photoluminescence quantum yields with optical methods are addressed, and challenges associated with these measurements are discussed. Special emphasis is dedicated to relative measurements of fluorescent (i.e., short excited-state lifetime), transparent, dilute dye solutions in conventional cuvettes in a 0°/90° measurement geometry. Recommendations on the selection of suitable quantum yield standards are presented, and requirements for the documentation of photoluminescence quantum yields are derived.