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Pure Appl. Chem., 2010, Vol. 82, No. 5, pp. 1161-1187

Published online 2010-04-20

Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

Jan Labuda1*, Ana Maria Oliveira Brett2, Gennady Evtugyn3, Miroslav Fojta4*, Marco Mascini5, Mehmet Ozsoz6, Ilaria Palchetti5, Emil Paleček4 and Joseph Wang7

1 Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia
2 Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-533 Coimbra, Portugal
3 Department of Analytical Chemistry, A.M. Butlerov Institute of Chemistry, Kazan State University, Kremlevskaya 18, 420008 Kazan, Russia
4 Institute of Biophysics, Academy of Sciences, Královopolská 135, 612 65 Brno, Czech Republic
5 Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy
6 Faculty of Pharmacy, Ege University, Bornova-Izmir, Turkey 35100
7 Department of Nanoengineering, University of San Diego, La Jolla, CA 92093, USA

Abstract: An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electrochemical NA biosensors, including their critical evaluation, which should be valuable for a wide audience, from academic, biomedical, environmental, and food-testing, drug-developing, etc. laboratories to sensor producers.