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Pure Appl. Chem., 2010, Vol. 82, No. 12, pp. 2315-2335

Published online 2010-11-02

Fluorescence standards: Classification, terminology, and recommendations on their selection, use, and production (IUPAC Technical Report)

Ute Resch-Genger1* and Paul C. DeRose2

1 Federal Institute for Materials Research and Testing (BAM), Richard-Willstaetter-Strasse 11, D-12489 Berlin, Germany
2 National Institute of Standards and Technology (NIST), 100 Bureau Drive, Gaithersburg, MD 20899-8312, USA

Abstract: Chromophore-based fluorescence standards for the characterization of photo-luminescence measuring systems and the determination of relevant fluorometric quantities are classified according to their scope and area of application. General and type-specific requirements for suitable standards are derived for each class of standards. Metrological requirements linked to the realization of comparable measurements are addressed and recommendations on selecting, using, and developing fluorescence standards are given.