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Pure Appl. Chem., 2008, Vol. 80, No. 12, pp. 2595-2608

Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm

Hiroki Haraguchi1, Atsushi Ishii1, Takuya Hasegawa1, Hirotaka Matsuura2 and Tomonari Umemura1

1 Department of Applied Chemistry, Graduate School of Engineering, Nagoya Universtiy, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
2 Department of Applied Chemisry and Biochemistry, Graduate School of Science and Technology, Kumamoto Univerity, 39-1, Kurokami 2-chome, Kumamoto 860-8555, Japan

Abstract: Salmon egg cells were the subject of investigation for all-elements analysis in a single biological cell, where the elements in egg cells were simultaneously determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) after microwave-assisted acid digestion. As a result, 74 elements among 78 stable isotope elements were determined or detected, although H, C, N, and O were determined separately by conventional CHN elemental analysis. In addition, the survey of protein-binding metallic elements as well as metalloid elements in salmon egg cell cytoplasm was performed by the fractionation analysis with a hyphenated system of high-performance liquid chromatography (HPLC)/ICP-MS using a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS)-coated octadecylsilyl silica (ODS) column and a size exclusion chromatography (SEC) column.