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Pure Appl. Chem., 2005, Vol. 77, No. 1, pp. 251-262

Production of a novel alkaline lipase by Fusarium globulosum using neem oil, and its applications

Ruchi Gulati1, Jasmine Isar1, Vineet Kumar2, Ashok K. Prasad2, Virinder S. Parmar2 and Rajendra K. Saxena1

1 Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
2 Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi 110 007, India

Abstract: A low-temperature, alkaline, and detergent-compatible lipase from Fusarium globulosum (FGL) has been produced using an inexpensive source, viz. neem oil. Maximum lipase production of 12 300 U/L was achieved in 96 h on optimizing various physicochemical parameters. The lipase exhibited remarkable stability in the presence of commercial detergents, bleaching agents, and proteases. The lipase showed a novel property of marked activation on prolonged incubation at alkaline pH. Wash performance analysis of the commercial detergent for removal of fatty stains improved upon addition of this lipase.