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Pure Appl. Chem., 2003, Vol. 75, No. 2-3, pp. 309-316

Exploitation of proteomics strategies in protein structure-function studies

Gennaro Marino1,2*, P. Pucci1,2, Leila Birolo2 and M. Ruoppolo1,3

1 CEINGE, Biotecnologie Avanzate, scrl, Napoli, Italy
2 Dipartimento di Chimica Organica e Biochimica, Facoltà di Scienze Biotecnologiche, Università di Napoli “Federico II”, Napoli, Italy
3 Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli “Federico II”, Napoli, Italy

Abstract: Mass spectrometry plays a central role in structural proteomics, particularly in highly intensive structural genomics projects. This review paper reports some examples taken from recent work from the authors' laboratory and is aimed at showing that modern proteomics strategies are instrumental in the integration of structural genomic projects in fields such as: (i) protein–protein interactions, (ii) protein-DNA interactions, (iii) protein–ligand interactions, and (iv) protein-folding intermediates.