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Pure Appl. Chem., 2000, Vol. 72, No. 9, pp. 1777-1781

Stereochemical support for Serratia endonuclease active-site geometry

W. J. Stec, M. Koziolkiewicz and K. Taira

Abstract: Earlier observation that stereoregular All-Rp-PS-oligonucleotides in the presence of nonsequence-specific Serratia endonuclease undergo nucleolytic degradation, confronted with recently published results on the active-site architecture of this enzyme, strongly supports an involvement of 3'-bridging and pro-Sp-nonbridging oxygen atoms of scissile internucleotide bond in the interactions with hydrated magnesium ion anchored by Asn-119 residue of this endonuclease.