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Pure Appl. Chem., 2010, Vol. 82, No. 1, pp. 313-319

Published online 2010-01-16

Auxin-producing Bacillus sp.: Auxin quantification and effect on the growth of Solanum tuberosum

Ambreen Ahmed1* and Shahida Hasnain2

1 Department of Botany, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan
2 Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan

Abstract: Plant-associated bacteria are known to improve plant growth and play a major role in the development of plants. The present study is concerned with the isolation of two auxin-producing plant growth-promoting bacteria (PGPB). On the basis of 16S rRNA sequencing, both of the strains are identified as Bacillus sp. Maximum auxin production was observed at 37 °C after 48 h of incubation. Increase in tryptophan concentration stimulated auxin production by the isolates. High-performance liquid chromatography analysis showed that the bacterial auxin exhibited similar retention time as the standard indole-3-acetic acid (IAA). Sprouts of Solanum tuberosum var. Desiree were inoculated with the isolates. Comparison of various growth parameters of inoculated plants with non-inoculated plants revealed the improvement of plant growth by bacterial inoculation. Almost 40 and 35 % increase in shoot length with P4 and S6 inoculation, respectively, was observed. Considerable improvement in root growth was observed with an increase in the number and length of roots. On the basis of the above findings, it is concluded that the plant growth-promoting Bacillus strains affect S. tuberosum beneficially, resulting in improved plant growth.