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Pure Appl. Chem., 2001, Vol. 73, No. 3, pp. 513-517

Protein dynamics detected by the time-resolved transient grating technique

Masahide Terazima

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan

Abstract: Dynamics of two proteins [myoglobin and photoactive yellow protein (PYP)] initiated by photoexcitation were studied by the time-resolved transient grating (TG) and photo-acoustic methods. For both proteins, the temporal profiles of the TG signals showed new features that have not been detected before by the transient absorption method. These dynamics were attributed to the volume change, which is caused by the protein dynamics apart from the chromophores.